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تاثیر تمرین تناوبی شدید بر بیان ژن پروتئین شوک گرمایی 70 و کاسپاز 3 کاردیومیوسیتهای موشهای نر مدل سکته قلبی. | ||
مطالعات کاربردی تندرستی در فیزیولوژی ورزش | ||
دوره 9، شماره 2، مهر 1401، صفحه 1-11 اصل مقاله (1.11 M) | ||
نوع مقاله: مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I | ||
شناسه دیجیتال (DOI): 10.22049/jahssp.2021.27521.1407 | ||
نویسندگان | ||
جبرییل پوزش جدیدی1؛ فرناز سیفی* 2؛ لطفعلی بلبلی3؛ آمنه پوررحیم3 | ||
1دانشجوی دکتری فیزیولوژی ورزش، گروه تربیت بدنی، دانشکده علوم تربیتی و روان شناسی، اردبیل، ایران | ||
2استادیار، گروه فیزیولوژی ورزش، دانشکده علوم تربیتی و روان شناسی، دانشگاه محقق اردبیلی، اردبیل، ایران.. | ||
3دانشیار، گروه فیزیولوژی ورزش، دانشکده علوم تربیتی و روانشناسی، دانشگاه محقق اردبیلی، اردبیل، ایران. | ||
چکیده | ||
هدف: هدف پژوهش بررسی تاثیر تمرین هوازی تناوبی شدید بر بیان ژن پروتئین شوک گرمایی 70 و کاسپاز 3 کاردیومیوسیتهای موشهای نر مدل سکته قلبی بود. روش شناسی: تعداد 30 سر موش صحرائی نر 16 هفتهای با وزن 200 تا 250 گرم نژاد ویستار با تزریق درون صفاقی ایزوپروترنول (با دوز 100 میلیگرم بر هر کیلوگرم وزن بدن در دو روز متوالی) دچار آنفارکتوس میوکارد (تایید از طریق شاخصهای آسیب قلبی: CK، LDH و CTnI) شدند و به طور تصادفی در سه گروه (10 سر موش در هر گروه) کنترل سالم، کنترل سکته، سکته + تمرین (HIIT+MI) تقسیم شدند. تمرین HIIT به مدت 8 هفته (5 روز در هفته) شامل 60 دقیقه دویدن تناوبی(4 دقیقه دویدن با شدت 90-85 درصد VO2max و 2 دقیقه ریکاوری فعال با شدت 60 – 50 درصد) انجام شد. بیان ژنهای HSP70 و کاسپاز 3 کاردیومیوسیتها با استفاده از روش Real-Time PCR انجام شد. دادهها با استفاده از روش تحلیل واریانس یک طرفه و آزمون تعقیبی توکی در سطح معنیداری 05/0 > pتحلیل شدند. یافتهها: بیان HSP70 کاردیومیوسیتها در موشهای کنترل مدل MI (سکته کنترل) نسبت به گروه سالم کنترل کمتر بود (001/0=p)؛ با این حال، بیان HSP70 در گروه تمرین HIIT به طور معنیداری بیشتر از گروه سکته کنترل و کنترل سالم بود (به ترتیب 001/0=p، 001/0=p). همچنین، بیان کاسپاز 3 در گروه تمرین HIIT به طور معنی داری کمتر از گروههای کنترل سالم و کنترل سکته بود (به ترتیب 001/0=p، 001/0=p). نتیجهگیری: به نظر میرسد تمرین تناوبی شدید در مهار آپوپتوز قلبی ناشی ناشی از سکته موثر باشد. اما، به دلیل محدودیتهای تحقیق و کمبود شواهد، هنوز نیاز به تحقیقات بیشتر باقی است. | ||
کلیدواژهها | ||
تمرین تناوبی شدید؛ پروتئین شوک گرمایی 70؛ سکته قلبی؛ کاسپاز 3 | ||
مراجع | ||
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