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تاثیر تمرین هوازی بر مقدار ادم پنجه و سطوح IL-6 ، TNF-α و CC16 سرم موشهای نر تحت مواجهه با آرسنیک | ||
مطالعات کاربردی تندرستی در فیزیولوژی ورزش | ||
دوره 9، شماره 1، فروردین 1401، صفحه 136-150 اصل مقاله (1.38 M) | ||
نوع مقاله: مقاله پژوهشی Released under (CC BY-NC 4.0) license I Open Access I | ||
شناسه دیجیتال (DOI): 10.22049/jahssp.2022.27769.1460 | ||
نویسندگان | ||
امیر داداش زاده؛ رقیه پوزش جدیدی* | ||
گروه فیزیولوژی ورزشی، دانشکده علوم انسانی، دانشگاه آزاد اسلامی واحد تبریز، تبریز، ایران. | ||
چکیده | ||
هدف: قرار گرفتن در معرض آرسنیک از طریق آب آشامیدنی یک مشکل بهداشت عمومی گسترده است که با افزایش خطر پیامدهای متعددی از جمله التهاب همراه است. هدف از این تحقیق، تعیین تاثیر تمرین هوازی بر مقدار ادم پنجه و سطوح IL-6 ، TNF-α و CC16 سرم موشهای نر تحت مواجهه با آرسنیک بود. روش شناسی: در این مطالعه تجربی، تعداد 32 سر موش صحرائی نر به چهار گروه شامل کنترل، آرسنیک، تمرین، و آرسنیک+تمرین تقسیم شدند. آرسنیک به مدت 8 هفته روزانه از طریق آب آشامیدنی با دوز پنج میلیگرم بر هر کیلوگرم وزن بدن به صورت گاواژ خورانده شد. تمرین به مدت هشت هفته با تکرار پنج روز در هفته، با سرعت 10 تا 25 متر در دقیقه، شیب 5 درصد و روزانه به مدت 30-10 دقیقه بر روی تردمیل ویژة جوندگان آزمایشگاهی انجام شد. از روشهای الایزا و حجم سنج پلتیسمومتر به ترتیب برای اندازه گیری سطوح IL-6 ، TNF-α و CC16 سرم و مقدار ادم پنجه پای موش استفاده شد. دادهها با روش تحلیل واریانس یک راهه و آزمون تعقیبی توکی در سطح معنیداری 05/0>p تحلیل شدند. یافتهها: مواجهه با آرسنیک، سبب افزایش سطوح IL-6 ، TNF-α و کاهش CC16 سرم موشهای نر شد (001/0=p برای هر سه متغیر)، اما بر ادم پنجه پای موش تاثیر معنیداری نداشت (8/0=p). مداخله تمرین در مقایسه با گروه کنترل، آرسنیک و آرسنیک+تمرین، افزایش سطوح IL-6 (به ترتیب 001/0=p، 02/0=p، 001/0=p)، TNF-α (به ترتیب 001/0=p، 02/0=p، 03/0=p) و کاهش CC16 سرمی (به ترتیب 03/0=p، 001/0=p، 008/0=p) ناشی از آرسنیک را افزایش داد. با اینحال، تمرین بر ادم پنجه پای موش تاثیر معنیداری نداشت (8/0=p). نتیجهگیری: در حالی که مواجهه با آرسنیک میتواند به افزایش سطوح IL-6، TNF-α و کاهش CC16 سرمی موشها منجر شود، به نظر می رسد که تمرین هوازی این پاسخهای التهابی را برعکس میکند. با این حال، به دلیل برخی محدودیتها و کمبود تحقیقات انسانی، به بررسیهای بیشتر نیاز است. | ||
کلیدواژهها | ||
آرسنیک؛ التهاب؛ ادم؛ CC16؛ تمرین هوازی | ||
مراجع | ||
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